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KMID : 1098420210290040282
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Korean Journal of Medicinal Crop Science
2021 Volume.29 No. 4 p.282 ~ p.292
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Development of Insertion or Deletion Markers to Distinguish Korean Jujube Cultivars
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Kim Moon-Kyo
Kim Ju-Hyeok
Lee Mi-Sun
Jo Nam-Su
Park Sang-Ik
Gil Jin-Su
Yeruult Enkhtsetseg
Oh Ha-Kyung
Lee Kyeong-Hee
Kim Ho-Bang
Lee Moon-Soon
Lee Yi
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Abstract
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Background: Jujube (Ziziphus jujuba Mill.) friuts are rich in nutrients and an economically and ecologically important medicinal plant in Korea. However, as it is difficult to distinguish jujube cultivars using morphological characters, the development of molecular markers is necessary to protect and distinguish Korean jujube cultivars.
Methods and Results: Next-generation sequencing analysis was performed on six major Korean cultivars; Bokjo, Boeun, Chuseok, Mudeung, Geumseong, and Wolchul. Sites with an insertion or deletion (InDel) were identified by comparison from the sequence information of the six Korean cultivars using the CLC Genomics Workbench. Among the identified InDels, we developed ZjInDel-1 and Zj-InDel-2 markers, which could distinguish Bokjo, Chuseok, and Boeun from the other Korean cultivars.
Conclusions: The InDel markers developed in this study could be used for classification of domestic jujube cultivars and protection of the elite ones.
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KEYWORD
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Ziziphus jujuba, Cultivar, Insertion or Deletion, Molecular Marker, Next-generation Sequencing
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